Figure 1
(A) Location of the studied ACTA1 mutations on the 3D-representation of the actin molecule [18]. Mutations indicated in green were biochemically analysed in this study, mutations indicated in pink were analysed in Costa et al. [12]. (B) Native gel analysis of twelve α-actin mutants causing NM. Autoradiograms of native gels of 35S-labeled WT α-actin and 12 mutants causing NM run with or without ATP. Actins were produced in in vitro transcription translation reactions in reticulocyte lysate, which endogenously contains CAP [22] and the actin folding machinery, prefoldin (PFD) and CCT [8, 9, 15]. The positions of these complexes with actin are indicated. The graph underneath indicates the percentage of actin in each of the bands (associated with CCT (purple), with prefoldin (white, (+ATP)) or CAP (white, (-ATP)) and free actin (red). In each lane the total actin is the sum of these three actin species.