Table 1 Strains and plasmids used in this study

Pseudomonas sp. B4 constitutive polyP(-)

Pseudomonas sp. B4 transfomant with pS7PPX1 plasmid

This study

Pseudomonas sp. B4 constitutive control

Pseudomonas sp. B4 transfomant with

pMLS7 plasmid

This study

Pseudomonas sp. B4 regulated polyP(-)

Pseudomonas sp. B4 transfomant with pBADPPX1 plasmid

This study

Pseudomonas sp. B4 regulated control

Pseudomonas sp. B4 transfomant with pMLBAD plasmid

This study

Escherichia coli NR100

M15 (pREP) derivative with pQE30PPK plasmid

[24]

Plasmids

pGEM^®-T-easy

T-vector cloning system

Promega

pMLBAD

Broad-host range regulated expression vector

[22]

pMLS7

Broad-host range constitutive expression vector

[22]

pTYPPX1

pGEM^®-T-easy with PPX1 gene from S. cerevisiae. (His6 tag)

This study

pS7PPX1

pMLS7 with PPX1 gene from S. cerevisiae. (His6 tag)

This study

pBADPPX1

pMLBAD with PPX1 gene from S. cerevisiae. (His6 tag)

This study