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Figure 1 | BMC Research Notes

Figure 1

From: Nucleotide-binding oligomerization domain containing 1 (NOD1) haplotypes and single nucleotide polymorphisms modify susceptibility to inflammatory bowel diseases in a New Zealand caucasian population: a case-control study

Figure 1

Infection with E. coli LF82 alters m RNA levels for Nod1 and NF-κB. Total RNA was isolated and cDNA synthesized by reverse transcription. Nod1 and NF-κB mRNA levels were assayed by real-time quantitative PCR using specific primers for these genes. cDNA levels were normalized using primers for Actin. Quantitative real time PCR reactions were performed in triplicate, and means and standard error plotted.

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