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Table 1 Sequence specific oligonucleotide used for PCR-amplification of HLA-A, B*46, DRB1

From: Identification of HLA-A*02-B*46 haplotype allele variant in Guangdong Han populations on the basis of PCR-SBT

Gene Names Primer Names Nucleotide sequence(5'-3') Tm° Position
HLA-A Aintron1F 5'-CTC TG(C/T) GGG GAG AAG CAA-3' 58 4864–4881a
  Aex5mod R 5'-CCA GCA A(G/T)G ATG CCC ACG AT-3' 62 6735–6754a
HLA-B BXI F 5'-GGG AGG AGC GAG GGG ACC (C/G)CA G-3' 74 5624–5645b
  BINT R 5'-GGA GGC CAT CCC CGG CGA CCT AT-3' 78 4703–4725b
HLA-DRB1*01 DRB01F 5'-TCC CAG TGC CCG CTC CCT-3' 62 -,*
  DRB01 R 5'-ACA CAC TCA GAT TCT CCG CTT-3' 62 -,*
HLA-DRB1*15,16 DRB02 F 5'-GGT GGG TGC TGT TGA AGG T-3' 60 -,*
  DRB02 R 5'-ACA CAC ACA CTC AGA TTC CCA-3' 62 -,*
HLA-DRB1*03 (exclude0317), 1402, 1406, 1413 DRB03 F 5'-AGC ACT AAG GAA GGG TTC AG-3' 60 -,*
  DRB03 R 5'-ACA CAC ACA CTC AGA TTC CCA-3' 62 -,*
HLA-DRB1*04 DRB04 F 5'-CCT GGG ATC AGA GGT AGA TTTT-3' 62 -,*
  DRB04 R 5'-ACA CAC ACA CTC AGA TTC TCC-3' 62 -,*
HLA-DRB1*07 DRB07 F 5'-CGG CGT CGC TGT CAG TGT T-3' 62 -,*
  DRB07 R 5'-TCA GAT TCC CAG CTC GGA GA-3' 62 -,*
HLA-DRB1*08 DRB08 F 5'-AGC GCA GGC CAG GCT CAA A T-3' 62 -,*
  DRB08 R 5'-ACA CAC ACA CTC AGA TTC CCA-3' 62 -,*
HLA-DRB1*09 DRB09 F 5'-CAG TTA AGG TTC CAG TGC CA-3' 60 -,*
  DRB09 R 5'-ACA CAC ACA CTC AGA TTC CCA-3' 62 -,*
HLA-DRB1*10 DRB10 F 5'-GGC GTT GCG GGT CGG CG-3' 62 -,*
  DRB10 R 5'-ACA CAC AGA GTC AGA TTC CCA-3' 62 -,*
HLA-DRB1*03(exclude0317), 11, 13, 14 DRB11.1 F 5'-TGG TGG GCG TTG GGG CG-3' 60 -,*
  DRB11.1 R 5'-ACA CAC ACA CTC AGA TTC CCA-3' 62 -,*
HLA-DRB1*11, 13(exclude1313), 14 DRB11.2 F 5'-AGC ACT AAG GAA GGG TTC AC-3' 60 -,*
  DRB11.2 R 5'-TGT CAC CTC CCC ACA GAG T-3' 60 -,*
HLA-DRB1*03, 11,13(exclude1313&0317) DRB11.3 F 5'-GTT TTC CCG CCT GGT CCC C-3' 64 -,*
  DRB11.3 R 5'-TGT CAC CTC CCC ACA GAG T-3' 60 -,*
HLA-DRB1*12 DRB12 F 5'-AAC AGG CTG GAG GTA CGG AC-3' 64 -,*
  DRB12 R 5'-ACA CAC ACA CTC AGA TTC CCA-3' 62 -,*
HLA-DRB1*1301,1302 DRB13.1 F 5'-GTG GGC GTT GCG GCG GC-3' 66 -,*
  DRB13.1 R 5'-ACA CAC ACA CTC AGA TTC CCA-3' 62 -,*
HLA-DRB1*14(exclude1402, 1406, 1413) DRB14 F 5'-GTT TTC CCG CCT GGA CCC T-3' 62 -,*
  DRB14 R 5'-ACA CAC ACA CTC AGA TTC CCA-3' 62 -,*
HLA-DRB1*07 DRB07 F 5'-CGG CGT CGC TGT CAG TGT T-3' 62 -,*
  DRB07 R 5'-TCA GAT TCC CAG CTC GGA GA-3' 62 -,*
HLA-DRB1*08 DRB08 F 5'-AGC GCA GGC CAG GCT CAA A T-3' 62 -,*
  DRB08 R 5'-ACA CAC ACA CTC AGA TTC CCA-3' 62 -,*
HLA-DRB1*09 DRB09 F 5'-CAG TTA AGG TTC CAG TGC CA-3' 60 -,*
  DRB09 R 5'-ACA CAC ACA CTC AGA TTC CCA-3' 62 -,*
HLA-DRB1*10 DRB10 F 5'-GGC GTT GCG GGT CGG CG-3' 62 -,*
  DRB10 R 5'-ACA CAC AGA GTC AGA TTC CCA-3' 62 -,*
  1. a, Numbering of the HLA-A sequence is from the GeneBank accession NT_007952
  2. b, Numbering of the HLA-B sequence is from the GeneBank accession NT_007592
  3. *, Numbering of the HLA-DRB1 sequence is from the reference 7 and http://www.ihwg.org