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Figure 4 | BMC Research Notes

Figure 4

From: A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells

Figure 4

Virus generation in 96-well format. (A) Percentage of EGFP positive wells as a sign of virus formation in the 96-well plates in 5 independent trials. (B) Flow cytometric analysis of the EGFP percentage in independent wells from 3 to 7 days post infection. (C) A representative well showing EGFP production in F-bacmid VEGF-DΔNΔC tranfected cells 7 days post transfection (10 × magnification). (D) Protein production in 96-well plates. Sf9 cells were infected with VEGF-DΔNΔC and avidin encoding F-bacmid viruses and infection was allowed to continue for 7 days. The upper immunoblot shows VEGF-DΔNΔC production and the lower immunoblot avidin production. (M) Marker (SeeBlue® PreStained Standard, Invitrogen).

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