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Figure 1 | BMC Research Notes

Figure 1

From: A SNaPshot assay for the rapid and simple detection of four common hotspot codon mutations in the PIK3CA gene

Figure 1

SNaPshot detection of four common hotspot mutations in the PIK3CA gene. (A-E) SNaPshot results obtained from the analysis of bladder tumor-derived cell line DNAs illustrating (A) wildtype, (B) E542K, (C) E545G, (D) E545K and (E) H1047R electropherogram patterns. (F) SNaPshot detection of an E542K codon mutation in DNA extracted from a formalin-fixed paraffin embedded seborrheic keratosis sample. Bases are represented by the following colours: A = green; C = black; G = blue; T = red. Orange peaks (S) represent the internal Genescan-120LIZ size standards. All DNAs were extracted using a QIAmp DNA kit.

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