SNaPshot detection of four common hotspot mutations in the PIK3CA gene. (A-E) SNaPshot results obtained from the analysis of bladder tumor-derived cell line DNAs illustrating (A) wildtype, (B) E542K, (C) E545G, (D) E545K and (E) H1047R electropherogram patterns. (F) SNaPshot detection of an E542K codon mutation in DNA extracted from a formalin-fixed paraffin embedded seborrheic keratosis sample. Bases are represented by the following colours: A = green; C = black; G = blue; T = red. Orange peaks (S) represent the internal Genescan-120LIZ size standards. All DNAs were extracted using a QIAmp DNA kit.