Table 1 Primers designed for SNP detection in the bovine TTN gene

TT-1F

5'-TGCAGCCAAAGATGTAGAAGC-3'

-7,203 to -7,183

60°C

TT-1R

5'-TCAGTCCTTCCAATGAATACCC-3'

-5,262 to -5,283

60°C

TT-2F

5'-GTCATGTATGGATGAGAGTTGGA-3'

-5,428 to -5,406

59°C

TT-2R

5'-GAGAGTCGGACACGACTGAA-3'

-3,487 to -3,506

59°C

TT-3F

5'-GCCATTAAATGTGTGCGTGA-3'

-3,618 to -3,599

60°C

TT-3R

5'-TTGTGCACGTGTGTGTATGG-3'

-1,674 to -1,693

60°C

TT-4F

5'-TTCCCATAAGAATTAAAGTGGTTTG-3'

-1,806 to -1,782

59°C

TT-4R

5'-GATAGCACCAAGTAGTCCGACA-3'

329 to 308

59°C

TT-5F

5'-CCTAAGAGGTGAGCAATCCA-3'

275,190 to 275,209

58°C

TT-5R

5'-GCTGTAGAGAACTTGAGTTGC-3'

276,756 to 276,736

58°C