Figure 1
From: Absence of GAPDH regulation in tumor-cells of different origin under hypoxic conditions in – vitro
GAPDH mRNA and protein expression under extreme hypoxic (0.1% O 2 ), normoxia and reoxygenation conditions in HepG2, Hep 1-6, Hep-3-B, A-549, HT-29 and HCT-116 cells, in vitro. (A) Semiquantitative RT-PCR analysis of GAPDH mRNA expression in HepG2, Hep 1-6 and Hep-3-B (B) Western blot expression analysis of GAPDH protein under identical conditions in HepG2, Hep 1-6 and Hep-3-B. (C) Semiquantitative RT-PCR analysis of GAPDH mRNA expression in A-549, HT-29 and HCT-116, in vitro (D) Western blot analysis of GAPDH protein expression under identical conditions in A-549, HT-29 and HCT-116. Representative experiments out of three for each experimental set.