Table 3 Expected and back-calculated (observed) concentrations in fmoles of Nanostring spike-in mixes 3 and 4, including %CV and %Bias.
Nanostring spike-in Mix #3 | Nanostring spike-in Mix #4 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Spike Name | Expected | Observed | StdDwev | CV, % | Bias, % | Expected | Observed | StdDev | CV, % | Bias, % |
S23 | 50 | 61.45 | 3.48 | 5.7 | 22.9 | 50 | 59.45 | 3.63 | 6.1 | 18.9 |
S14 | 5 | 3.36 | 0.28 | 8.5 | 32.9 | 5 | 3.24 | 0.31 | 9.5 | 35.2 |
S19 | 0.5 | 0.42 | 0.07 | 17.2 | 16.5 | 0.5 | 0.45 | 0.08 | 17.3 | 10.6 |
S8 | 5 | 3.19 | 0.24 | 7.5 | 36.2 | 15 | 9.94 | 0.62 | 6.3 | 33.7 |
S13 | 15 | 10.74 | 0.48 | 4.5 | 28.4 | 5 | 3.08 | 0.31 | 10.0 | 38.5 |
S22 | 5 | 4.72 | 0.30 | 6.3 | 5.5 | 50 | 56.19 | 3.77 | 6.7 | 12.4 |
S7 | 50 | 53.23 | 2.67 | 5.0 | 6.5 | 5 | 4.36 | 0.30 | 7.0 | 12.9 |
S17 | 1.5 | 2.41 | 0.25 | 10.4 | 60.8 | 4.5 | 7.96 | 0.75 | 9.4 | 76.9 |
S3 | 4.5 | 5.4 | 0.33 | 6.0 | 19.9 | 1.5 | 1.62 | 0.19 | 11.8 | 7.8 |
S6 | 0.25 | 0.31 | 0.07 | 21.8 | 23.3 | 0.75 | 1.02 | 0.13 | 12.8 | 35.7 |
S4 | 0.75 | 1.06 | 0.13 | 12.1 | 41.5 | 0.25 | 0.38 | 0.07 | 17.7 | 50.4 |