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Table 3 Expected and back-calculated (observed) concentrations in fmoles of Nanostring spike-in mixes 3 and 4, including %CV and %Bias.

From: Multiplexed measurements of gene signatures in different analytes using the Nanostring nCounter™ Assay System

  Nanostring spike-in Mix #3 Nanostring spike-in Mix #4
Spike Name Expected Observed StdDwev CV, % Bias, % Expected Observed StdDev CV, % Bias, %
S23 50 61.45 3.48 5.7 22.9 50 59.45 3.63 6.1 18.9
S14 5 3.36 0.28 8.5 32.9 5 3.24 0.31 9.5 35.2
S19 0.5 0.42 0.07 17.2 16.5 0.5 0.45 0.08 17.3 10.6
S8 5 3.19 0.24 7.5 36.2 15 9.94 0.62 6.3 33.7
S13 15 10.74 0.48 4.5 28.4 5 3.08 0.31 10.0 38.5
S22 5 4.72 0.30 6.3 5.5 50 56.19 3.77 6.7 12.4
S7 50 53.23 2.67 5.0 6.5 5 4.36 0.30 7.0 12.9
S17 1.5 2.41 0.25 10.4 60.8 4.5 7.96 0.75 9.4 76.9
S3 4.5 5.4 0.33 6.0 19.9 1.5 1.62 0.19 11.8 7.8
S6 0.25 0.31 0.07 21.8 23.3 0.75 1.02 0.13 12.8 35.7
S4 0.75 1.06 0.13 12.1 41.5 0.25 0.38 0.07 17.7 50.4
  1. Calculations based on all replicates.
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