Figure 1
From: Purification and characterization of a serine protease (CESP) from mature coconut endosperm
Purification of the protease (CESP) from coconut endosperm. The crude extract after ammonium sulfate fractionation was subjected to (A), Gel filtration chromatography; (B), Phenyl sepharose affinity chromatography; (C), DEAE-cellulose chromatography; (D), Arginine-Sepharose affinity chromatography; (E), Biogel P60 gel filtration chromatography. "Black circles" represents the absorbance of the protein at 280 nm and "white circles" represents the protease activity in Enzyme Units (EU). The straight line in figures B, C and D represents the gradient of salt concentration used for eluting the protease.