Table 1 Summary of Purification of CESP from coconut kernel.
From: Purification and characterization of a serine protease (CESP) from mature coconut endosperm
Steps | Total Protein (mg) | Total Activity (EU) | Specific Activity (EU/mg) | Yield (%) | Fold Purification |
|---|---|---|---|---|---|
1. Crude extract | 1380 | 38.17 | 0.028 | 100 | 1 |
2. Sephadex G 200 | 16.51 | 19.09 | 1.16 | 50.02 | 41.42 |
3. Phenyl-Sepharose | 1.36 | 5.95 | 4.37 | 15.57 | 156.1 |
4. DEAE-Cellulose | 0.986 | 4.86 | 4.93 | 12.74 | 176.1 |
5. Arginine-Sepharose | 0.260 | 3.21 | 12.37 | 8.43 | 441.8 |
6. Biogel P60 | 0.183 | 2.59 | 14.42 | 6.78 | 515 |