Skip to main content

Table 2 Hydrolysis of fluorogenic peptide substrate by the purified CESP

From: Purification and characterization of a serine protease (CESP) from mature coconut endosperm

Fluorogenic Substrates Concentration
used (mM)
Relative Fluorescence
Intensity
Boc-Leu-Arg-Arg-MCA 0.05 698.00
Boc-Val-Pro-Arg-MCA 0.05 583.50
Suc-Ala-Ala-Phe-MCA 0.05 120.70
Suc-Leu-Leu-Val-Tyr-MCA 0.05 0.060
Boc-Phe-Ser-Arg-MCA 0.05 625.85
Bz-Phe-Val-Arg-βNA 0.05 422.90
Bz-Leu-Leu-Glu-βNA 0.05 0.020
  1. 100 μl of reaction mixture containing 10 μl of purified enzyme, 0.05 mM of the substrates and 100 mM Tris HCl pH 8.5 was incubated at 37°C for 30 min and the reaction was stopped by the addition of 1.9 ml of ethanol. The product of hydrolysis was measured spectroflourimetrically (for AMC-Exc-380 nm, Em-460 nm and for βNA-Exc-335 nm, Em-410 nm).