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Table 3 Effect of protease inhibitors, metal ions, chelators and sulfhydryl reagents on the activity of the purified CESP

From: Purification and characterization of a serine protease (CESP) from mature coconut endosperm

Inhibitor

Conc. (mM)

Enzyme

Activity (%)

Ions/Complex

Enzyme

Activity (%)

No addition

-

100

No addition

100

TLCK

0.541

0

Ca

106

TPCK

0.568

13

Mg

115

Antipain

0.073

11

Zn

102

Benzamidine

0.641

51

Cu

79

PMSF

1.000

57

DTT

109

SBTI

0.0025

97

β-ME

103

Pepstatin

0.0364

93

EDTA

100

Aprotinin

0.0077

100

EGTA

103

  1. The enzyme activity of purified CESP was measured in presence of inhibitors using 0.5 mM BAPNA in a reaction mixture (500 μl) as described earlier. The 100% activity is represented by 0.015 EU.