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Table 3 Effect of protease inhibitors, metal ions, chelators and sulfhydryl reagents on the activity of the purified CESP

From: Purification and characterization of a serine protease (CESP) from mature coconut endosperm

Inhibitor Conc. (mM) Enzyme
Activity (%)
Ions/Complex Enzyme
Activity (%)
No addition - 100 No addition 100
TLCK 0.541 0 Ca 106
TPCK 0.568 13 Mg 115
Antipain 0.073 11 Zn 102
Benzamidine 0.641 51 Cu 79
PMSF 1.000 57 DTT 109
SBTI 0.0025 97 β-ME 103
Pepstatin 0.0364 93 EDTA 100
Aprotinin 0.0077 100 EGTA 103
  1. The enzyme activity of purified CESP was measured in presence of inhibitors using 0.5 mM BAPNA in a reaction mixture (500 μl) as described earlier. The 100% activity is represented by 0.015 EU.