The proximal first exon architecture of the murine ghrelin gene is highly similar to its human orthologue

BMC Research Notes

Table 1 Designations and sequences of primers used in RT-PCR

Name	Sequence (5'-3')	Ghrl Exon	Ta (°C)	PCR Cycles
RACEout-F	AATTCGTCACTCCGTGAATCAG	N/A
RACEout-R	CAGAGCATGCTGAGTAGCAG	1	62	25
RACEin-F	GTCACTCCGTGAATCAGATCG	N/A
RACEin-R	CAGCAAACTGCAGATGGTG	1	61	35
Ext1-F	AAGGCACATAACATGGAGATGAAG	1*
Ex1-R	CTTGGTGGTGAGGACAGATGAC	1	60	40
Ex4-R	GCCTGTCCGTGGTTACTTGT	4	58	40
Gapdh-F	ACCTGCCAAGTATGATGACATCA	N/A
Gapdh-R	GGTCCTCAGTGTAGCCCAAGAT	N/A	60	40

Annealing temperatures (Ta) of oligonucleotide primers employed in RT-PCR are shown. The location of oligonucleotide primers spanning ghrelin exons are listed, while oligonucleotides spanning synthetic sequences (adapters and linkers) or genes other than Ghrl are denoted as N/A (not applicable). The novel extended exon 1 is denoted as 1*. All primers were synthesised by Proligo (Armidale, Australia).

ISSN: 1756-0500