Table 2 Primers used in this study for real time RT-PCR and genetic deletions
Target | Forward primer | Reverse primer |
|---|---|---|
ACT1 | 5'-GCCATTTTGAGAATCGATTTG-3' | 5'-TTAGAAACACTTGTGGTGAAC-3' |
ECM4 | 5'-GTGGTACAAACGGAGCTTTCA-3' | 5'-GTGCCCAATGGACTACGCTACA-3' |
SPI1 | 5'-CCAGAACCAACGACTTTCGTA-3' | 5'-ACTGCACCAGCCAAACCTA-3' |
ROM1 | 5'-AGCTATCTACGCCTCCAACT-3' | 5'-ATGATGACGTTGGTGTTGA-3' |
SLT2 | 5'-AGCAACAGCAGCCTTCAGA-3' | 5'-GAACGCGAGGAAGTATCCAA-3' |
YHR097C | 5'-CCATCGTCGTACATCACAC-3' | 5'-GTACAGGCGCCACTTTATTA-3' |
SLT2 pRS | 5'-AATGGAAAGTTTCAGTGTTAAAAATAG AAACTGAAAAAGGAGATCTAGCCCGTTTCGGTGATGAC-3' | 5'-AATAATGAATATTGTCTATAGATGACTAA TCATAAATGAACGAAAAGAAATTCCTGATGCGGTATTTTCTCC-3' |
ATG9 pRS | 5'-TTACACTAATTAAGATGCTTAGATTCC CATTCAAAAGGTACTATTGACGTCGTTTCGGTGATGAC-3' | 5'-GGCGGAAAGAAATAACCAATAATAA TAATTTATTAACCTCTTTTTTTCTTTTCCT GATGCGGTATTTTCTCCT-3' |