Table 4 List of primers for the 12 conserved SSRs used for wet-lab experiment
From: Orthology between genomes of Brachypodium, wheat and rice
Brachypodium contig Id. | Motif | Primer sequence 5'-3' | Tm (°C) | Product size (bp) | Gene class |
|---|---|---|---|---|---|
BDEST01P1_Contig9 | (gct)5 | L GCCTATGTTTCCGCAGAGAG R CCAGGCAAGAAGTTCCTGAG | 59.98 | 203 | Formate dehydrogenase |
BDEST01P1_Contig1223 | (cca)4 | L AGCCAACTCTTGCAGCAAAT R TGTTGCTCCCTCCTTTTGAT | 59.67 | 207 | Endo-1,4-beta-glucanase Cel1 |
BDEST01P1_Contig1335 | (tca)4 | L GACGAGAGGTTGTGTTGGTG R ACAGGACACCGTCAGAGGAA | 60.71 | 239 | Putative uncharac-terized protein |
BDEST01P1_Contig1574 | (cgc)6 | L CAAAACCCTAGCTGCCCTTC R TGCCAGTGCTTCTTGAAATG | 59.99 | 123 | Putative 60S ribos-omal protein L13E |
BDEST01P1_Contig2089 | (ggc)4 | L GCTCTTCTCGCCCCTCTACT R CTCCATCTGGAAATCGCAGT | 60.22 | 200 | Hypothetical protein |
BDEST01P1_Contig2416 | (tcaaga)2 | L CCGCACCTCAAGGACTACA R TCGGAGGAGATCTTGGTGAG | 60.34 | 194 | Succinate dehydrogenase |
BDEST01P1_Contig2648 | (ggt)4 | L AAACCACTTGCCAAAACACC R GCTGCGGTTCTCCATGAC | 60.37 | 248 | Putative uncharac-terized protein |
BDEST01P1_Contig3139 | (tcg)4 | L AGTCACCAAGGTCGTCAAGG R CCTTCGCTGCTCCATAGTCT | 59.6 | 224 | Putative ribosomal protein |
BDEST01P1_Contig3247 | (tggtgc)2 | L AGTTGGAATGAGGGCATCAG R TTCAAGGCTCTCGAGTAGGG | 59.57 | 214 | Putative uncharac-terized protein |
BDEST01P1_Contig3321 | (gctcgc)2/N36/(ggc)4 | L CACTTCGAGTTTCCCGTCAT R TTTTGCAGTGTCCACACCAT | 60.01 | 244 | Protein disulfide isomerase 2 precursor |
BDEST01P1_Contig3721 | (gct)4 | L GGACTACTTTGGGGCTCACA R GGATTCATAACTGGCAACCA | 59.44 | 180 | Cytosolic 6-phosphogluconate dehydrogenase |
BDEST01P1_Contig3747 | (tcgcca)2 | L AGGTCAACTCGGTCAACGAC R AGGTCAGCCCGTTGTTGTAG | 60.17 | 192 | Phenylalanine ammonia-lyase |