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Table 1 Primer sequences.

From: An insertion in the coding region of the myostatin (MSTN) gene affects carcass conformation and fatness in the Norwegian Spælsau (Ovis aries)

Name

Direction

Position

Sequence 5'-3'

F1083:

Forward

-128 to -109a

5'-TCACTGGTGTGGCAAGTTGT-3'

R0997:

Reverse

716–735b

5'-TCCTGGTTCTGGGAAGGTTA-3'

F0790:

Forward

528–547b

5'-CATCAAACCCATGAAAGACG-3'

R1566:

Reverse

1285–1304a

5'-GGTTAAATGCCAACCATTGC-3'

MAF1:

Forward

81–100b

5'-CGAGCAGAAGGAAAATGTGG-3'

MAF2:

Reverse

149–168b

5'-TATGGCTTCTAGTCTTGAGG-3'

MAE1:

Reverse

118–140b

5'-TTTTGTCTCCACAAGCATGCATT-3'

  1. a, Positions are numbered according to first base of the translation start codon of the bovine MSTN gene (NM_001001525.2).
  2. b, Positions are numbered according to first base of the translation start codon of the sheep MSTN gene (AM992883).