Effect of RNA interference on short circuit current of SCCD epithelium. Effect of α-ENaC siRNA on dexamethasone stimulated Na+ transport: Epithelia were incubated with dexamethasone (100 nM) for 24 hr concurrently with transfection. Amiloride-sensitive short circuit current, AMIL-I
, was measured from both transfected and non-transfected monolayers as a function of net Na+ transport. α-ENaC siRNA (anti-sense) but not non-silencing siRNA (sense) inhibits dexamethasone-stimulated Na+ transport. "Control" epithelia were not incubated with dexamethasone. Data are mean ± SEM, n = 3 - 10. P < 0.05 (*) compared to AMIL-I
with dexamethasone alone. Note the break in the vertical axis.