Figure 2

Evidence of infection by PCR. PCR confirmation that cultures of CHSE-214 and RTS11 had been infected with either P. salmonis (A) or IPNV (B). P. salmonis was revealed by primer specific amplification of the ITS region of the bacteria 16S operon (A) and IPNV by primer specific amplification of the VP2 fragment (B). These results rule out persistency of infection in control samples, as they showed no primer specific amplification.