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Table 1 Subtraction efficiencies of human genomic DNA using three different types of probes

From: Effect of probe characteristics on the subtractive hybridization efficiency of human genomic DNA

Concentration

(ng/μl)

Subtraction efficiency

(%) PCR probes

Subtraction efficiency

(%) Sequenase probes

Subtraction efficiency

(%) Klenow probes

12

66 ± 6

88 ± 3

85 ± 4

6

80 ± 1

77 ± 25

94 ± 5

3

82 ± 6

93 ± 6

95 ± 5

  1. The subtractions were performed in duplicate experiments.