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Table 1 Validation of pure cultures by single and double-locus detection assays

From: Evaluation of single and double-locus real-time PCR assays for methicillin-resistant Staphylococcus aureus (MRSA) surveillance

  Real Time PCR results
Pure culture strains
Total n = 150
Cuny's[7]
single-locus detectiona
Huletsky's[8]
single-locus detectionb
Reischl's[5]
double-locus detectionc
  SCC:orfX+
(MRSA)
SCC:orfX-
(other)
SCC:orfX+
(MRSA)
SCC:orfX-
(other)
Sa442+ mec+
(MRSA)
Sa442+ mec-
(MSSA)
Sa442- mec+
(Oxa. resistant)
Sa442- mec-
(other)
MRSA (n = 50) 49 1 49 1 50 --- --- ---
MSSA (n = 50) 14 36 2 48 --- 48 --- 2
MR-CoNS (n = 25) --- 25 --- 25 --- --- 25 ---
MS-CoNS (n = 25) --- 25 --- 25 --- --- --- 25
  1. a sensitivity = 98%, specificity = 86%, Positive predictive value = 77.8%, Negative predicted value = 98.9%
  2. b sensitivity = 98%, specificity = 98%, Positive predictive value = 96.1%, Negative predicted value = 99%
  3. c sensitivity = 100%, specificity = 100%, Positive predictive value = 100%, Negative predicted value = 100%