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Table 1 Enzymatic characterization

From: Expression, purification, and initial characterization of different domains of recombinant mouse 2',3'-cyclic nucleotide 3'-phosphodiesterase, an enigmatic enzyme from the myelin sheath

 

CNPase construct

Lee et al.[19]

 

1-1 (500 ng)

2-1 (5 ug)

3-1 (500 ng)

3-2 (500 ng)

Full-length

Catalytic fragment

Km (μM)

193 ± 26

537 ± 14

445 ± 41

406 ± 11

263 ± 12

295 ± 22

Kcat (s-1)

270 ± 10

118 ± 1.1

570 ± 19

612 ± 6.1

836 ± 11

1690 ± 39

Kcat/Km

1.40

0.22

1.28

1.51

3.2

5.7

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BMC Research Notes

ISSN: 1756-0500

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