Detection of RNA extracted with different methods, Poly(A)+ RNA and the cDNA quality using agarose gel electrophoresis. Total RNA from jatropha immature seeds: (A) RNA extracted with method I(Lane i), RNA extracted with method II (Lane ii) and RNA extracted with Qiagen RNA Mini Kit (Lane iii). (B) Poly(A)+ RNA purified from the total RNA using modified method II. (C) Double strand cDNA (ds cDNA) after size fractionation with column chromatography. The RNA and 1-kb DNA marker were from New England Biolabs. The image is representative of four independent experiments.