Evaluation of jatropha seed cDNA library. Poly(A)+ RNA was purified from total RNA extracted with method II and used for cDNA library construction using CloneMiner™ cDNA Library Construction Kit (Invitrogen). Plasmid DNA of 20 positive clones was digested with BsrG 1 enzyme (New England Biolabs) and electrophoresed on 1% agarose gel to determine average insert size of cDNA. (Lane 1) 1-kb DNA marker (New England Biolabs); (lane 2) vector pDONR™ 222 (Invitrogen); (Lanes 3-22) randomly picked cDNA clones. Band at size 2.5 Kb is the vector backbone. cDNA insert of the clone in lane 3 has similar size as that of vector backbone, which did not separate in this gel electrophoresis.