Figure 3From: An improved method for RNA isolation and cDNA library construction from immature seeds of Jatropha curcas LNorthern hybridization of jatropha immature seed RNA with Ketoacyl ACP reductase (KAR) probe. RNA extracted with method I and II from jatropha leaves and seeds was fractionated on 1.2% agarose-formaldehyde denaturing gel and blotted onto Hybond-N+ nylon membrane. Stained blot was photographed and then hybridized with Ketoacyl ACP reductase (KAR) probe labelled with [32P]-dCTP. The hybridized bands were detected by exposing filters to KODAK Biomax MS Autoradiography Film at -80°C. The image is representative of four independent experiments.Back to article page