Northern hybridization of jatropha immature seed RNA with Ketoacyl ACP reductase (KAR) probe. RNA extracted with method I and II from jatropha leaves and seeds was fractionated on 1.2% agarose-formaldehyde denaturing gel and blotted onto Hybond-N+ nylon membrane. Stained blot was photographed and then hybridized with Ketoacyl ACP reductase (KAR) probe labelled with [32P]-dCTP. The hybridized bands were detected by exposing filters to KODAK Biomax MS Autoradiography Film at -80°C. The image is representative of four independent experiments.