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Table 1 RNA yield and quality detected with spectrophotometer1

From: An improved method for RNA isolation and cDNA library construction from immature seeds of Jatropha curcas L

Sample Method2 A260/280 A260/230 28S:18S RNA yield (μg)/FW3 (g) Time
Jatropha I 1.98 ± 0.03 1.91 ± 0.02 1.49 ± 0.21 124.30 ± 8.82 2 d
  II 2.14 ± 0.02 2.25 ± 0.04 1.73 ± 0.08 282.42 ± 12.91 3 h
Mustard I 1.85 ± 0.04 1.90 ± 0.04 1.24 ± 0.08 189.95 ± 5.00 2 d
  II 2.05 ± 0.04 2.32 ± 0.01 1.68 ± 0.18 240.55 ± 11.36 3 h
Rice I 1.94 ± 0.03 1.95 ± 0.02 1.55 ± 0.22 254.90 ± 12.74 2 d
  II 2.00 ± 0.03 2.20 ± 0.02 1.85 ± 0.03 335.35 ± 17.36 3 h
  1. 1Based on 4 individual samples
  2. Method I, CTAB method with LiCl precipitation
  3. 2Method II, combination of CTAB based total RNA extraction method and RNeasy® Plant Mini Kit (Qiagen, Germany).
  4. FW, fresh weight,
  5. 3Based on Nanodrop readings.