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Table 1 RNA yield and quality detected with spectrophotometer1

From: An improved method for RNA isolation and cDNA library construction from immature seeds of Jatropha curcas L

Sample

Method2

A260/280

A260/230

28S:18S

RNA yield (μg)/FW3 (g)

Time

Jatropha

I

1.98 ± 0.03

1.91 ± 0.02

1.49 ± 0.21

124.30 ± 8.82

2 d

 

II

2.14 ± 0.02

2.25 ± 0.04

1.73 ± 0.08

282.42 ± 12.91

3 h

Mustard

I

1.85 ± 0.04

1.90 ± 0.04

1.24 ± 0.08

189.95 ± 5.00

2 d

 

II

2.05 ± 0.04

2.32 ± 0.01

1.68 ± 0.18

240.55 ± 11.36

3 h

Rice

I

1.94 ± 0.03

1.95 ± 0.02

1.55 ± 0.22

254.90 ± 12.74

2 d

 

II

2.00 ± 0.03

2.20 ± 0.02

1.85 ± 0.03

335.35 ± 17.36

3 h

  1. 1Based on 4 individual samples
  2. Method I, CTAB method with LiCl precipitation
  3. 2Method II, combination of CTAB based total RNA extraction method and RNeasy® Plant Mini Kit (Qiagen, Germany).
  4. FW, fresh weight,
  5. 3Based on Nanodrop readings.