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Figure 2 | BMC Research Notes

Figure 2

From: 1Identification of genes differentially expressed in the embryonic pig cerebral cortex before and after appearance of gyration

Figure 2

Verification of microarray data with qRT-PCR. Nine genes differently expressed from the microarray analysis were examined for the expression level using mRNA extracted from pig cortical tissue from E60 and E80. qRT-PCR analysis were performed on cDNA for the genes GFAP, ApoE, calbindin-2, Neurofilament (200 kDa), S100A1, TUBA1, Neurogranin, ACTN2, CHN1, and Dbx17. The expression levels were normalized to GAPDH, Beta-actin and 18S rRNA expression using the geNorm program [30].

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