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Figure 3 | BMC Research Notes

Figure 3

From: Cloning of NruI and Sbo13I restriction and modification sstems in E. coli and amino acid sequence comparison of M.NruI and M.Sbo13I with other amino-methyltransferases

Figure 3

Dot blot analysis of M.NruI and M.Sbo13I modified genomic DNA using rabbit antibodies against N6mA-methylated DNA (left panel) or N4mC-methylated DNA (right panel). M.EcoRI and M.ApoI are known N6mA methylases; while M.BamHI, M.BglIII and possibly M.BstYI are N4mC methylases. Negative control total DNAs were prepared from transformants of empty vectors. Three DNA dilutions (150 ng, 75 ng, and 25 ng) were spotted on the membrane for detection.

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BMC Research Notes

ISSN: 1756-0500

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