Development of genome-specific primers for homoeologous genes in allopolyploid species: the waxy and starch synthase II genes in allohexaploid wheat (Triticum aestivum L.) as examples

Table 2 Classification of the partial waxy wheat lines according to the presence (+) or absence (-) of each waxy protein and amplification results of each genome-specific primer set.

Genotype	Accession number	Country	Null allele	Type*	Wx protein			Genome-specific primer set
					Wx-A1	Wx-B1	Wx-D1	Wx-7A-F1R1a	Wx-4A-F2R2	Wx-7D-F3R3a
Chinese Spring	-	China	Wild type	1	+ ^†	+	+	+	+	+
Sturdy	CItr 13684	USA	null (Wx-A1b)	2	-	+	+	-	+	+
Fujimi Komugi	PI 360869	Japan	null (Wx-A1b)	2	-	+	+	-	+	+
Gabo	PI 155431	Australia	null (Wx-B1b)	3	+	-	+	+	-	+
Gamenya	PI 268329	Australia	null (Wx-B1b)	3	+	-	+	+	-	+
Santanta	CItr 14582	USA	null (Wx-B1b)	3	+	-	+	+	-	+
Bai Huo	PI 606717	China	null (Wx-D1b)	4	+	+	-	+	+	-
Kanto 107	PI 631445	Japan	null (Wx-A1b & B1b)	7	-	-	+	-	-	+
NSGC 8645	PI 612545	USA	Triple null alleles	8	-	-	-	-	-	-
NSGC 8646	PI 612546	USA	Triple null alleles	8	-	-	-	-	-	-

ISSN: 1756-0500