The time delay linear regression model of the HO gene in wild type cells. (A) The expression pattern of HO and its regulators ASH1, TEC1, and SWI5. The solid lines represent interpolated values based on experimentally observed values (X symbol), and the black dotted line represents the values of the target gene fitted by the model. The colored dotted lines represent the regulatory contribution of each regulator; the black dotted line is the sum of these. The time delays of ASH1, TEC1, and SWI5 are 23.5, 29.4, and 29.4 minutes, respectively. Before 95.6 min, the cell cycle phase is recovering from synchrony; from 95.6 to 107.5 min, the phase is 1st cycle G1; from 107.5 to 122.9 min, the phase is 1st cycle S; from 122.9 to 172.5 min, the phase is 1st cycle G2/M; from 172.5 to 184.4 min, the phase is 2nd cycle G1; from 184.4 to 199.8 min, the phase is 2nd cycle S; from 199.8 to 249.3 min, the phase is 2nd cycle G2/M. (B) Cell cycle diagram of corresponding model. The target gene HO and its regulators are arranged on the basis of the time of peak transcript levels.