SUMO-1 DNA interaction detected using NMR. (A) Comparison of 1H projections of the 15N-1H HSQC spectra of 15N-SUMO-1 (black) and 15N-SUMO-1 at 20 μM with 135 μM of 25-mer double-stranded DNA containing a G:U mismatch (red). (B) SUMO-1 (PDB ID 2ASQ) ribbon representation in grey. SUMO-1 residues implicated in DNA binding are colored as a function of their chemical shift variations: red for Δδ > 0.06 ppm and orange for 0.02 < Δδ < 0.06 ppm. (C) SUMO-1 solvent accessible surfaces colored as described for B.