(A) Size distribution of the 46,462 inserts generated by the shotgun technique and of the 4,489 CDSs annotated in the XAC genome. (B) Distribution of the 4,421 selected clones, which best represented 3,084 CDSs of the XAC genome. Note in both figures that for the CDSs that were 0 to 200 bp in length and for a small fraction of genes that were up to 400 bp in length, representative clones were not found in the shotgun library, following the selection criteria described in the methods. (C) Representative scheme of the type of clones selected from the XAC genomic library. The blue region represents the sequence of the pUC19 cloning vector. The region marked by dotted vertical lines bounds the insert of the clone. The region where the CDSs do not overlap is marked in yellow, and the corresponding overlap is marked in red. The region with a serrated end represents the rupture of the sequence of the gene of interest. Black arrows represent the universal oligonucleotide primer (M13) used for the PCR amplification of the insert.