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Table 2 Analysis of the CDSs deposited on the XACarray based on the hybridization signal during the experimental validation of the platform.

From: Development and validation of a Xanthomonas axonopodis pv. citri DNA microarray platform (XACarray) generated from the shotgun libraries previously used in the sequencing of this bacterial genome

Type of clones

Physical analysis (Fixed PCR products)

Annotationc

Hybridization signal

 

Clones

n° (%)

AISa

(bp)

C/Ib

(%)

Function

n° (%)

Hypothetical

n° (%)

Function

n° (%)

Hypothetical

n° (%)

I

1,184 (57.6)

1,487

56.8

631 (53.2)

553 (46.8)

574 (90.9)

485 (87.7)

II

292 (63.1)

1,121

100

235 (80.5)

57 (19.5)

215 (91.5)

52 (91.2)

III

490 (59.3)

1,211

69.9

343 (70.0)

147 (30.0)

323 (94.2)

123 (83.7)

IV

673 (59.9)

1,209

70.5

466 (69.2)

207 (30.8)

436 (93.6)

184 (88.9)

Total

2,639 (59.7)

1,324

82.1

1,675 (63.5)

964 (36.5)

1,521 (90.8)

844 (87.6)

      

2,365 (89.6)

  1. a AIS (average of insert size).
  2. b C/I (average of overlapping size CDS/Insert)
  3. c Annotation based on Silva et al 2002 [6].
  4. Note that the number of clones that were amplified and positively annotated represent only 59.7% of the total clones initially selected (4,421), and that 2,365 of individual CDSs presented a positive hybridization signal, which represent 52.7% of the total annotated CDSs in the genome and 89.6% of the fixed products.