Skip to main content

Table 2 Analysis of the CDSs deposited on the XACarray based on the hybridization signal during the experimental validation of the platform.

From: Development and validation of a Xanthomonas axonopodis pv. citri DNA microarray platform (XACarray) generated from the shotgun libraries previously used in the sequencing of this bacterial genome

Type of clones Physical analysis (Fixed PCR products) Annotationc Hybridization signal
  Clones
n° (%)
AISa
(bp)
C/Ib
(%)
Function
n° (%)
Hypothetical
n° (%)
Function
n° (%)
Hypothetical
n° (%)
I 1,184 (57.6) 1,487 56.8 631 (53.2) 553 (46.8) 574 (90.9) 485 (87.7)
II 292 (63.1) 1,121 100 235 (80.5) 57 (19.5) 215 (91.5) 52 (91.2)
III 490 (59.3) 1,211 69.9 343 (70.0) 147 (30.0) 323 (94.2) 123 (83.7)
IV 673 (59.9) 1,209 70.5 466 (69.2) 207 (30.8) 436 (93.6) 184 (88.9)
Total 2,639 (59.7) 1,324 82.1 1,675 (63.5) 964 (36.5) 1,521 (90.8) 844 (87.6)
       2,365 (89.6)
  1. a AIS (average of insert size).
  2. b C/I (average of overlapping size CDS/Insert)
  3. c Annotation based on Silva et al 2002 [6].
  4. Note that the number of clones that were amplified and positively annotated represent only 59.7% of the total clones initially selected (4,421), and that 2,365 of individual CDSs presented a positive hybridization signal, which represent 52.7% of the total annotated CDSs in the genome and 89.6% of the fixed products.