Skip to main content

Table 1 DNA fragment profile (expected and observed) after amplification with Felid 16S rRNA primers and restriction digestion.

From: An evaluation of the PCR-RFLP technique to aid molecular-based monitoring of felids and canids in India

Species

GenBank Accession numbers

Taq I

Hae III

Ase I

Nla III

Pac I

Dpn I

Jungle cat

(Felis chaus)

AF006393.1

1

2

2

1

3

1

Caracal

(Caracal caracal)

AF006389.1

1

2

1

1

1

1

Leopard cat

(Prionailurus bengalensis)

AF006437.1

2

1

2

2

2

1

Fishing cat

(Prionailurus viverrinus)

AF006451.1

2

1

2

1

2

1

Rusty-spotted cat (Prionailurus rubiginosus)

AF006445.1

1 (2)*

2 (1)*

1 (2)*

1

2

1

Lynx

(Lynx lynx)

AF006413.1

AY499288.1

AY499289.1

AY499290.1

AY499291.1

2

1

1

1

2

2

Manul

(Otocolobus manul)

AF006431.1

1

2

1

2

2

1

Marbled cat

(Pardofelis marmorata)

AY499300.1

AF006439.1

1

2

1

1-2#

2

1

Asian golden cat (Catopuma temminkii)

AF006447.1

1

2

1

1

2

2

Domestic cat

(Felis silvestris catus)

AF006453.1

1

2

1

1

2

1

Wild cat grp.

(Felis silvestris)

AF006395.1

AF006401.1

1

2

1

1

2

1

Clouded leopard

(Neofelis nebulosa)

AF006425.1

AY499301.1

2

1

1

1-2# (2)*

2

2

Lion

(Panthera leo)

AF006457.1

FJ151641.1

FJ151644.1

FJ151652.1

2

1

1

2

2

2

Tiger

(Panthera tigris)

EF394928.1

EF392683.1

EF551003.1

AY452110.1

2

2

2

2

2

1

Leopard

(Panthera pardus)

AF006443.1

DQ904388.1

EU223367.1

2

1

1

1-2# (2)*

2

3

Snow leopard

(Uncia uncia)

AF006449.1

EF551004.1

1

1

1

2

2

2

  1. * Figures in brackets indicate our results that were different from what was expected.
  2. # Two or more sequences from GenBank with differing restriction digestion patterns.