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Table 1 DNA fragment profile (expected and observed) after amplification with Felid 16S rRNA primers and restriction digestion.

From: An evaluation of the PCR-RFLP technique to aid molecular-based monitoring of felids and canids in India

Species GenBank Accession numbers Taq I Hae III Ase I Nla III Pac I Dpn I
Jungle cat
(Felis chaus)
AF006393.1 1 2 2 1 3 1
Caracal
(Caracal caracal)
AF006389.1 1 2 1 1 1 1
Leopard cat
(Prionailurus bengalensis)
AF006437.1 2 1 2 2 2 1
Fishing cat
(Prionailurus viverrinus)
AF006451.1 2 1 2 1 2 1
Rusty-spotted cat (Prionailurus rubiginosus) AF006445.1 1 (2)* 2 (1)* 1 (2)* 1 2 1
Lynx
(Lynx lynx)
AF006413.1
AY499288.1
AY499289.1
AY499290.1
AY499291.1
2 1 1 1 2 2
Manul
(Otocolobus manul)
AF006431.1 1 2 1 2 2 1
Marbled cat
(Pardofelis marmorata)
AY499300.1
AF006439.1
1 2 1 1-2# 2 1
Asian golden cat (Catopuma temminkii) AF006447.1 1 2 1 1 2 2
Domestic cat
(Felis silvestris catus)
AF006453.1 1 2 1 1 2 1
Wild cat grp.
(Felis silvestris)
AF006395.1
AF006401.1
1 2 1 1 2 1
Clouded leopard
(Neofelis nebulosa)
AF006425.1
AY499301.1
2 1 1 1-2# (2)* 2 2
Lion
(Panthera leo)
AF006457.1
FJ151641.1
FJ151644.1
FJ151652.1
2 1 1 2 2 2
Tiger
(Panthera tigris)
EF394928.1
EF392683.1
EF551003.1
AY452110.1
2 2 2 2 2 1
Leopard
(Panthera pardus)
AF006443.1
DQ904388.1
EU223367.1
2 1 1 1-2# (2)* 2 3
Snow leopard
(Uncia uncia)
AF006449.1
EF551004.1
1 1 1 2 2 2
  1. * Figures in brackets indicate our results that were different from what was expected.
  2. # Two or more sequences from GenBank with differing restriction digestion patterns.