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Table 2 DNA fragment profile (expected and observed) after amplification with Canid 16S rRNA primers and restriction digestion.

From: An evaluation of the PCR-RFLP technique to aid molecular-based monitoring of felids and canids in India

Species

GenBank Accession numbers

Bsm I

Xsp1/Bfa I

Ssp 1

Ase I

Nla III

Domestic dog

(Canis lupus familiaris)

EU789728.1

EU789759.1

EU789784.1

1

2

1

1

1 (2)*

Gray wolf

(Canis lupus)

AM711902.1

DQ480507.1

DQ480508.1

1

2

1

1

1-2# (1)*

Himalayan wolf

(Canis lupus chanco)

EU442884.2

AY289963.1

GQ374438.1

1

1-2# (1)*

1

1

1

Golden jackal

(Canis aureus)

AY289969.1

AY289970.1

2

1

1

1

1

Asiatic Wild dog

(Cuon alpinus)

AY289971.1

AY289972.1

GU063864.1

1

1

2

1

1

Red fox

(Vulpes vulpes)

AM181037.1

GQ374180.1

DQ334815.1

1

1

1

2

1

Bengal fox

(Vulpes bengalensis)

 

(1)

 

(2)

(2)

(1)

  1. * Figures in brackets indicate our results that were different from what was expected.
  2. # Two or more sequences from GenBank with differing restriction digestion patterns.