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Table 3 Measured unbound bilirubin (Bf) concentrations at various ratios of [UCB]/[BSA]

From: Effect of bilirubin on cytochrome c oxidase activity of mitochondria from mouse brain and liver

  Bf (nM)   
UCB/BSA Ratio, [BSA] μM Controla BMb LMc p -value
(BM vs LM)
p -value
(control vs LM)
0.20, 60 22.3 ± 3.4 22.6 ± 2.2 30.8 ± 2.2 p < 0.005 p < 0.005
0.35, 60 61.4 ± 2.3 59.6 ± 2.0 61.5 ± 4.3 p = 0.215, NS p = 0.788, NS
0.50, 60 90.2 ± 2.6 93.4 ± 5.6 104 ± 4.6 p < 0.005 p < 0.005
0.75, 30 141 ± 3.9 142 ± 3.4 150 ± 6.3 p < 0.005 p < 0.005
  1. Bf concentrations were determined by the peroxidase method in potassium phosphate buffer (50 mM KH2PO4 - 50 mM K2HPO) containing 50 mM KCl, pH 7.4 [22, 23]. aControl, no mitochondria, bBM, mouse brain mitochondria; cLM, mouse liver mitochondria. Data are expressed as mean ± SD of at least 9 experiments.