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Table 3 Measured unbound bilirubin (Bf) concentrations at various ratios of [UCB]/[BSA]

From: Effect of bilirubin on cytochrome c oxidase activity of mitochondria from mouse brain and liver

 

Bf (nM)

  

UCB/BSA Ratio, [BSA] μM

Controla

BMb

LMc

p -value

(BM vs LM)

p -value

(control vs LM)

0.20, 60

22.3 ± 3.4

22.6 ± 2.2

30.8 ± 2.2

p < 0.005

p < 0.005

0.35, 60

61.4 ± 2.3

59.6 ± 2.0

61.5 ± 4.3

p = 0.215, NS

p = 0.788, NS

0.50, 60

90.2 ± 2.6

93.4 ± 5.6

104 ± 4.6

p < 0.005

p < 0.005

0.75, 30

141 ± 3.9

142 ± 3.4

150 ± 6.3

p < 0.005

p < 0.005

  1. Bf concentrations were determined by the peroxidase method in potassium phosphate buffer (50 mM KH2PO4 - 50 mM K2HPO) containing 50 mM KCl, pH 7.4 [22, 23]. aControl, no mitochondria, bBM, mouse brain mitochondria; cLM, mouse liver mitochondria. Data are expressed as mean ± SD of at least 9 experiments.
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BMC Research Notes

ISSN: 1756-0500

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