Table 1 Heat-shock response vs. sex in bovine fibroblast cells carrying the Hsp70.1Luciferase transgenes
Origin of the fibroblast cultures | Sex | Mean luciferase specific activitya RLU.μg protein-1.min-1 ± SD | Immunostained luciferase-positive cells after heat shock b | |
|---|---|---|---|---|
Control | After heat shock | |||
Transgenic adult bull "OV7060" | Male (XLUCY) | 205 ± 76 | 2.58 × 106 ± 0.32 × 106 | 92% |
Transgenic fetus "BSF731" | Female (XLUCX) | 315 ± 79 | 0.99 × 106 ± 0.13 × 106 | 80% |
Transgenic fetus "F616" | Female (XLUCX) | 875 ±160 | 2.62 × 106 ± 0.35 × 106 | nd |