Heat-induced and spontaneous expression of Hsp70.1Luciferase transgene copies localized on Xp22 in female bovine cells

Table 3 Changes in luciferase activity and in the percentage of luciferase-positive embryos during early development

Embryo type		Stage
		1-2-cell	4-8-cell	8-cell	16-cell	Morula	Blasto.
Female IVF	Number of embryos	36	20	24	30	32	28
	Luciferase activity (RLU. min^-1. embryo^-1)	325 ± 175*	70	20940 ± 5790	36540 ± 8900	3000 ± 1880	370 ± 330
	% positive embryos	11%^a	5% ^a	50% ^b	47% ^b	28% ^ab	7% ^a
Male SCNT	Number of embryos	-	13	27	33	9	12
	Luciferase activity (RLU. min^-1. embryo^-1)	-	0	33500 ± 7056	13700 ± 3120	1400 ± 446	480 ± 165
	% positive embryos	-	0% ^a	85% ^b	97% ^b	67% ^c	17% ^a
Female SCNT	Number of embryos	-	20	21	39	10	8
	Luciferase activity (RLU. min^-1. embryo^-1)	-	515 ± 140	2240 ± 300	9915 ± 1490	525 ± 337	245
	% positive embryos	-	16% ^a	86% ^b	100% ^b	40% ^a	13% ^a

^abc different letters indicate that values on the line are significantly different (P < 0.05).
* SEM; SEM could not be calculated when 0 or only one embryo displayed a level of luciferase activity above the background level.

ISSN: 1756-0500