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Table 1 Scores for in silico selection.

From: A general framework for optimization of probes for gene expression microarray and its application to the fungus Podospora anserina

Criteria Measure Score values per criterion
   0 1 4 20
CHI % identity 60% 61- 84% ≥ 85% NAa
Self-folding structure ΔG > -8 kcal/mol ≤ -8 kcal/mol NAa NAa
Probe position in CDS Nucleotides numbered from CDS 3' end 1-500 500 - 1000 > 1000 NAa
Probe position relative to intron Classes defined in Figure 1 Class 1 Class 2 Class 3 NAa
Sequence match Used after genome re-annotation Perfect match NAa NAa Mismatch
  1. a Not Attributed