Table 1 Scores for in silico selection.

CHI

% identity

60%

61- 84%

≥ 85%

NA^a

Self-folding structure

ΔG

> -8 kcal/mol

≤ -8 kcal/mol

NA^a

NA^a

Probe position in CDS

Nucleotides numbered from CDS 3' end

1-500

500 - 1000

> 1000

NA^a

Probe position relative to intron

Classes defined in Figure 1

Class 1

Class 2

Class 3

NA^a

Sequence match

Used after genome re-annotation

Perfect match

NA^a

NA^a

Mismatch