Figure 1
From: Endothelial cells stimulate growth of normal and cancerous breast epithelial cells in 3D culture
Endothelial cells stay quiescent but metabolically active when cultured within rBM. When BRENCs are seeded onto rBM they form network structures within a few hours that disassociate from the rBM within 72 hours (upper panel). In contrast BRENCS that are embedded into rBM (lower panel) stay quiescent but metabolically active for a prolonged time in culture. Fluorescent staining in a and c reveals CD31 expression. Note the insert in (d) that demonstrates the uptake of Ac-LDL at day 14. Bars = 50 μm.