Figure 1

Example of Exon Coverage of Optimus Primer Designed Amplicons. Image from the UCSC genome browser showing Optimus Primer design results for the gene TCF7L2 [10]. The black boxes (highlighted by the red arrow) indicate the positions of all isPCR validated amplicons designed by OP. Note that only the gene name, TCF7L2, was submitted into the pipeline yet amplicons were designed spanning the exons (indicated by blue boxed) for all 6 known isoforms of the gene.