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Table 1 Example PCR Primer Pairs Designed After Submitting TCF7L2

From: Optimus Primer: A PCR enrichment primer design program for next-generation sequencing of human exonic regions

Region Name

Forward Primer

Reverse Primer

TCF7L2_chr10_exon1-3_1

CTGTTTATTTATGCACACGTCACTG

gTACTCACCTTCTTCCAAACTTTCC

TCF7L2_chr10_exon1-3_2

GATTCTTTTTCTCCCCCTTCTC

AGCCAGCAATCTCCACTAGAAAG

TCF7L2_chr10_exon4_1

TCCCTGTATGCTTATTGAATAGGTG

CCCAGAAAAGAAGTCATGGAATAAC

TCF7L2_chr10_exon5_1

GGCTAGTTGTTCTGCATTTACTTTC

AGCTCCAAAATAAGGAGGCAGTTAG

TCF7L2_chr10_exon6_1

AATAGGAGAGTTCTGATTTGTGCTG

GACTTATTCCACATTTTGTCTCTCC

TCF7L2_chr10_exon7_1

CATCTTAGAAAATCCAGGTGAGAGG

AACAACTGGGATAAAAAGGGGATG

TCF7L2_chr10_exon8_1

ATGAGATGAAACCTACCAACAACTC

TGTTGTTCAGAGTACAGATCACTGG

TCF7L2_chr10_exon9_1

CTTACTGTGCAGAGAGAACTTTTCC

ATTAGCGACTAAAACATACTGCTGC

TCF7L2_chr10_exon10-12_1

ACGATTTACACAGCTTTCTGTCTTC

CTATGTCATTCTGTCATTTGCTCAC

TCF7L2_chr10_exon10-12_2

CTGAGTGCACGTTGAAAGAAAG

AGAGAGGACTAGGCAGATCCTGTAG

TCF7L2_chr10_exon13-14_1

CAGACACTCTTCTCACATCTGTTTC

GGAGGTTTATTACTGAATTCCTTCC

TCF7L2_chr10_exon15-16_1

CTCGCTTCTCTCTTGAACTCATTC

CTTCTCCATGTGTCTCGACTCTAAG

TCF7L2_chr10_exon17_1

TATTCACAGATAACTCTCTCCCCTG

TCTATTAAGTGTTGAGTAGCGTCCC

TCF7L2_chr10_exon17_2

ACATCTGGTTTTTAAACCGTAAGGG

GAGCATAAAACGGAACAGTAACATC

TCF7L2_chr10_exon17_3

CATATTACATACGAGTAGGCAGCAG

CGATACTGTGGTCACCTTAAGAAAC

  1. After having only submitted the gene name (TCF7L2) Optimus Primer was able to successfully design PCR primers to amplify all exons (see figure 1 for amplicons position). Note that exon in close proximity to each other were merged into single amplicons (exons 1-3 for example) and that large exons were divided into several amplicons to maintain consistent amplicons size (exon 17 for example).