Time and dose dependant propofol induced (A) c-Fos and (B) Egr-1 transcription in brain slices. Transcription of c-Fos and Egr-1 was quantified using qRT-PCR. Gene transcription is normalized to actin and non-treated controls. Data are presented as mean ± sd from three independent experiments. * p < 0.05; ** p < 0.01 versus non-treated control.