Figure 2

TFII-I expression in head region at E9 (A-D) and at E12 (E, F, G). A. Head region (5 ×), B. Optic vesicle (32 ×), C. Neural tube (64 ×), D. Brain region (40 ×), E-F. Brain Region (32 ×), G. Nasopharynx (40 ×). TFII-I is more discretely localized at ED9 with nuclear localization. A. At low power, TFII-I immunoreactivity is visible in multiple regions of the embryo, but is most intense in the neural tube. B. The developing eye demonstrates TFII-I expression in the inner and outer retinal layer as well as lens primordium. C and D. Neural tube demonstrates intense nuclear immunoreactivity in individual cells. At E12 TFII-I is expressed extensively in brain, in contrast to TFII-IRD1, whereas TFII-I is only moderately present in nasopharynx. E. TFII-I is expressed in both nuclear and cytoplasmic localization in brain (32 ×). F. TFII-IRD1 antibody demonstrates no immunoreactivity in an adjacent section. G. Nasopharynx also demonstrates predominantly cytoplasmic localization (40 ×). Inset in Figure 2D shows nuclear and cytoplasmic locatization of TFII-I under 100 × magnification (Zeiss Axioscop, obj. Apochromat 100 ×). White arrows indicate positive reaction of specific antibody with TFII-I. Abbr.: CM-Cephalic Mesenchyme, CS-Cardiovascular System, FCC - Future Cerebral Cortex, IL-Inner Retinal Layer, IR-Intra-Retinal Space, LP-Lens Primordium, M-Mesenchyme, NE-Neuroepithelium, NL-Neural Lumen, NT-Neural Tube, OE-Olfactory Epithelium, OL-Outer Retinal Layer, OP-Optic Pit, PN- Primitive Nasopharynx,