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Table 1 Primers used for semi-quantitative RT-PCR

From: Expression of pathogenesis related genes in response to salicylic acid, methyl jasmonate and 1-aminocyclopropane-1-carboxylic acid in Malus hupehensis (Pamp.) Rehd

name

Forward Primer Sequence

[5'-3']

Reverse Primer Sequence

[5'-3']

Ampicon size

PR1

CCACAAAGAGAACAAACCATTAAC

GACCAACGCCTACTGCTG

160

PR5

CATGTCCTCCCACAGAGTAC

ATATAATCCCATTTCGTGCTTATG

153

PR8

AGCAGGTTCTATGACAACGG

CGCCATCATCCCTAACACAC

112

tubulin

AGGATGCTACAGCCGATGAG

GCCGAAGAACTGACGAGAATC

192

  1. The primers were set using the software beacon designer 7.0 and were synthesized at Shanghai Invitrogen Biotechnology Co., Ltd.