Figure 3

Destroying of the actin cytoskeleton does not affect flow-mediated activation of ENaC. (A) Summary graph of inhibition extent of amiloride-sensitive current. The peak response of the whole-cell amiloride-sensitive current at basal conditions was normalized to the flow-stimulated currents. All three mENaC subunits were overexpressed in CHO cells and ENaC activity recorded either with or without perfusion. CHO cells were not treated or pretreated with Cytochalasin D (CytD, 10 μg/ml) or Latrunculin B (LatB, 5 μM) for 20 min and 1-2 hrs, respectively. The number of observations for each group is shown. (B) Summary graph of the time constants describing the rate of ENaC inhibition following switching off flow stimulation of ENaC activity in CHO cells not treated and pretreated with CytD or LatB as presented in (A).