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Table 3 Primer sequences, purpose, annealing temperature (TA), number of bands and product size for primers during PCR, accession number and the linkage groups (LG) where the fragments were localized in Arctic charr

From: Clock genes and their genomic distributions in three species of salmonid fishes: Associations with genes regulating sexual maturation and cell cycling

Primer Name Primer Sequence (5'→3')   TA # of Bands Band size Sequence Name (GB Accession #) Family & parent LG
Clock1a-1 F-CCCAGTGTCCAGGTGAGAAA Mapping 57    3♀ 16
  R-CCTTTGTTCAGGGACACATT       
Clock1b F-ACCCAGAATACAGGGCAGAAC Sequencing 51 2 SalClock1b   
  R-CAGGATCAATTGAGTGGACTGG    1.5 kb, 1.3 kb (GU228524)   
Clock1b-3 F-GAGTACTGCCCTGCAGGTTG Mapping 55    3♂ 13
  R-TTGACCATGGCCCTCTTATG       
NPAS2-3 F-CTTATAATCTCCACCCAGCACA Mapping 61    2♀ 43
  R-GTCCGTGACAGAGTGCGATA      3♂ 20
amh F-ACTCCTCCATGTGGTGCAA Mapping     2♀ 13
  R-CCATACAGTCTTATCTGATCATGC      3♀ 13