Clock genes and their genomic distributions in three species of salmonid fishes: Associations with genes regulating sexual maturation and cell cycling

Table 3 Primer sequences, purpose, annealing temperature (TA), number of bands and product size for primers during PCR, accession number and the linkage groups (LG) where the fragments were localized in Arctic charr

Primer Name	Primer Sequence (5'→3')		T_A	# of Bands Band size	Sequence Name (GB Accession #)	Family & parent	LG
Clock1a-1	F-CCCAGTGTCCAGGTGAGAAA	Mapping	57			3♀	16
	R-CCTTTGTTCAGGGACACATT
Clock1b	F-ACCCAGAATACAGGGCAGAAC	Sequencing	51	2	SalClock1b
	R-CAGGATCAATTGAGTGGACTGG			1.5 kb, 1.3 kb	(GU228524)
Clock1b-3	F-GAGTACTGCCCTGCAGGTTG	Mapping	55			3♂	13
	R-TTGACCATGGCCCTCTTATG
NPAS2-3	F-CTTATAATCTCCACCCAGCACA	Mapping	61			2♀	43
	R-GTCCGTGACAGAGTGCGATA					3♂	20
amh	F-ACTCCTCCATGTGGTGCAA	Mapping				2♀	13
	R-CCATACAGTCTTATCTGATCATGC					3♀	13

ISSN: 1756-0500