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Figure 2 | BMC Research Notes

Figure 2

From: Role of DNA methylation in miR-200c/141 cluster silencing in invasive breast cancer cells

Figure 2

The activity of the promoter is regulated by DNA methylation. (A) TaqMan-based RT-PCR analysis of miR-200c and miR-141 in MDA-MB-231 kept under standard conditions and treated with 5-AZA-CdR at two different concentrations. Data are mean of triplicates and were normalized according to the ΔΔCt method using miR-16 as normalizer gene. (B) Firefly luciferase gene reporter assays of the miR-200c/141 promoter region in either demethylated or methylated states, respectively on Hela cells.

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