Figure 3

Predominant inclusion of NOD2 exon 3a from the rs2067085 C allele and concomitant splicing to exon 3. Sequencing of RT-PCR amplicons from human leukocyte cDNA to elucidate the processing of the primary transcript in the regions of exons 2-3a and 3a-4 as well as 2-3 and 3-4. Fluorescence traces are given for the SNP context in exon 2 (rs2067085, minor allele frequency 0.388), exon 3a (rs34133110) and exon 4 (rs2066842, minor allele frequency 0.362). Line: primary transcript; boxes: exons, arrows: PCR primers; dotted lines: amplifiable region; angulated lines: observed splice events.