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Figure 5 | BMC Research Notes

Figure 5

From: NOD2-C2 - a novel NOD2 isoform activating NF-κB in a muramyl dipeptide-independent manner

Figure 5

MDP-independent activation of NF-κB by NOD2-C2. Relative NF-κB activity was measured using a luciferase-based NF-κB reporter assay. HEK293 cells were cotransfected with NOD2-C2 (A), NOD2 or NOD2-C2/NOD2 (B) expression constructs and dual-lucifease reporter plasmid. 10 μg/ml MDP were used to stimulate NOD2-mediated NF-κB activation. Relative luciferase activity (compared to NOD2 (2ng) without MDP stimulation) is plotted against NOD2 and/or NOD2-C2 plasmid amounts. Values represent the mean ± standard deviation of triplicate cultures. Statistically significant differences are marked by asterisk * p < 0.05; ** p < 0.01.

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